30/07/2019
Marie Skłodowska-Curie Actions

MSCA-IF: SITE DIRECTED MUTAGENESIS TO PREVENT INSULIN RESISTANCE IN NEURODEGENERATIVE


  • OFFER DEADLINE
    30/06/2020 14:30 - Europe/Brussels
  • EU RESEARCH FRAMEWORK PROGRAMME
    H2020 / Marie Skłodowska-Curie Actions
  • LOCATION
    Spain, Castellón de la Plana
  • ORGANISATION/COMPANY
    Universitat Jaume I
  • DEPARTMENT
    Medicine
  • LABORATORY
    Neurobiotechnolgoy

  • Contact Person / Scientist in charge

Ana María Sánchez-Pérez

ORCID number 0000-0002-5811-0005

  • Brief description of the Research Group

The Neurobiotechnology group focuses on the study of the molecular mechanisms underlying neurodegenerative and developmental disorders. Specifically, our laboratory has been interested in the insulin and IGF1 actions in the brain. The group has developed AAV expressing shRNA against two major Insulin receptors substrates (IRS1 and IRS2) and study the specific effect in behaviour of IRS1 and IRS2 knockdown in specific neurons (manuscripts in preparation). In addition, we have been evaluating the beneficial effect of nutraceutical Abscisic acid (ABA, PPARγ agonists) that improve insulin signalling in the context of neuroinflammation models (ie high fat diet-fed rat model and murine Alzheimer model).

Our group carries out routinely cell culture and molecular techniques (cloning, qPCR), behavioural and immunological techniques (immunohistochemistry, western blot).

  • Brief description of the university

The Universitat Jaume I in Castelló (UJI), founded in 1991, is the public university serving the north of the Valencian Community (Spain). With almost 30 years of history, the UJI ranks among the 601- 800 best universities in the world, according to the World University Rankings 2018, conducted by The Times Higher Education, that places it in 12th place out of the 38 participating Spanish universities and the second in the Valencian Community.

Ensuring the researchers can enjoy of their rights and obligations as well as boosting researchers' careers, the UJI has recently applied for the HR Excellence in Research Award in order to align our human resources policies to the principles of the European Charter & Code of Conduct for the Recruitment of Researchers.

The university has services to support research, such as the Central Scientific Instrumentation Service (SCIC), a prestigious Library, the Animal Experimentation Service (SEA), the Ethics Committeethe Scientific Culture and Innovation Unit (UCC + i), the Office for Cooperation in Research and Technological Development (OCIT) as well as the European & International Projects Office completely devoted to the management of research projects such as the Marie Skłodowska-Curie Actions (MSCA-IF).

  • Project description

The project involves site-directed mutagenesis. The aim is to mutate proteins in specific codons and the study of the functional effects of such amino acid changes in vitro and to evaluate behavioural alterations derived from mutated proteins in the context of neuroinflammation. In addition, resultant mutant proteins will be expressed in the bacterial expression system.

The candidate is required to have a strong background in bioinformatics and molecular biology (cloning PCR). The candidate will be expected to work in a team, actively participating in seminars and journal clubs. Co-directing master theses and/or PhD theses is optional (and recommended).

  • Research area

Life Sciences

 

  • Applications (documents to be submitted):

  • CV

  • Name and contact mail address of two referees

  • Motivation letter

Disclaimer:

The responsibility for the hosting offers published on this website, including the hosting description, lies entirely with the publishing institutions. The application is handled uniquely by the employer, who is also fully responsible for the recruitment and selection processes.